Abstract：
:Microsatellite sequences are important markers for population genetics studies. In the past, the development of adequate microsatellite primers has been cumbersome. However with the advent of next-generation sequencing technologies, marker identification in genomes of non-model species has been greatly simplified. Here we describe microsatellite discovery on a Pacific Biosciences single molecule real-time sequencer. For the Greater White-fronted Goose (Anser albifrons), we identified 316 microsatellite loci in a single genome shotgun sequencing experiment. We found that the capability of handling large insert sizes and high quality circular consensus sequences provides an advantage over short read technologies for primer design. Combined with a straightforward amplification-free library preparation, PacBio sequencing is an economically viable alternative for microsatellite discovery and subsequent PCR primer design.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Grohme MA,Soler RF,Wink M,Frohme Mdoi
10.2144/000114104subject
Has Abstractpub_date
2013-11-01 00:00:00pages
253-6issue
5eissn
0736-6205issn
1940-9818pii
000114104journal_volume
55pub_type
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