Abstract：
:Comprehensive analysis of DNA methylation patterns is critical for understanding the molecular basis of many human diseases. While hundreds of PCR-based DNA methylation studies are published every year, the selection and implementation of appropriate methods for these studies can be challenging for molecular genetics researchers not yet familiar with methylation analysis. Here we review the most commonly used PCR-based DNA methylation analysis techniques: bisulfite sequencing PCR (BSP), methylation specific PCR (MSP), MethyLight, and methylation-sensitive high resolution melting (MS-HRM). We provide critical analysis of the strengths and weaknesses of each approach as well as a series of guidelines to assist in selecting and implementing an appropriate method.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Hernández HG,Tse MY,Pang SC,Arboleda H,Forero DAdoi
10.2144/000114087subject
Has Abstractpub_date
2013-10-01 00:00:00pages
181-97issue
4eissn
0736-6205issn
1940-9818pii
000114087journal_volume
55pub_type
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