Abstract：
:A general method for solid-phase gene assembly on streptavidin-coated magnetic beads has been developed. The introduction of biotin in the 5'-end of the initiation oligonucleotide enables anchoring to the bead by means of the streptavidin-biotin interaction. The immobilization of one oligonucleotide enables controlled, stepwise annealing/ligation of successive 5'-phosphorylated oligonucleotides to rapidly build up predesigned gene constructs. In this report, we have assembled gene constructs of different lengths derived from the Plasmodium falciparum malaria blood-stage antigen Ag332. The encoded gene products were subsequently expressed in Escherichia coli using two parallel expression systems based on staphylococcal protein A and streptococcal protein G, respectively.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ståhl S,Hansson M,Ahlborg N,Nguyen TN,Liljeqvist S,Lundeberg J,Uhlén Msubject
Has Abstractpub_date
1993-03-01 00:00:00pages
424-34issue
3eissn
0736-6205issn
1940-9818journal_volume
14pub_type
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