Abstract:
:Phage display holds a key position in the use of combinatorial library approaches for the purpose of protein engineering and discovery. However, modifying the pIII protein of the phage can severely and negatively influence the infectiousness of the phage particle. This concern is particularly relevant when large pIII fusions in combination with multivalent display systems are in use. We here describe the use of trypsin to restore wild-type pIII phenotype as a small modification to the standard titration protocol. The results show that the trypsin treatment has a very large but heterogeneous effect on the phage infection efficiency, depending on the pIII fusion domain and the valence of display.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Løset GÅ,Kristinsson SG,Sandlie Idoi
10.2144/000112724subject
Has Abstractpub_date
2008-04-01 00:00:00pages
551-2, 554issue
4eissn
0736-6205issn
1940-9818pii
000112724journal_volume
44pub_type
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