Abstract:
:A simple, nondestructive PCR-based screening method has been developed for identifying putative transgenic soft white winter wheat (Triticum aestivum L.) carrying the coat protein gene of wheat streak mosaic virus. Removal of the endosperm end of individual seed provided sufficient material for DNA extraction and PCR. DNA from seed is more free of the secondary, metabolites found in leaf tissue that can inhibit both PCR and restriction digests required for Southern analysis. The half-seed PCR assay has comparable accuracy to the leaf-tissue PCR assay and hence can be used as an accurate and rapid method for identifying transformed lines before planting. Germination of the remaining seed portion showed germination rates comparable to whole-seed controls. A slight delay in growth from the first-leaf through the first-tiller stage was observed in the half-seed-derived plants, as compared to plants grown from whole seed.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
McCarthy PL,Hansen JL,Zemetra RS,Berger PHdoi
10.2144/02323st09subject
Has Abstractpub_date
2002-03-01 00:00:00pages
560, 562-4issue
3eissn
0736-6205issn
1940-9818journal_volume
32pub_type
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