Abstract：
:A simplified method for producing recombinant baculovirus for expression of foreign genes is described. The method utilizes insect cells infected with the wild-type virus before transfection with the plasmid transfer vector, instead of the standard procedure utilizing cotransfection with a plasmid and viral DNA. Recombinant virus is preselected by a limiting dilution dot-blot hybridization procedure, rather than by morphologic criteria alone. In addition, we have found that plasmid purification by anion-exchange chromatography is as efficacious for transfection as plasmid purified by cesium chloride density gradient centrifugation. These modifications allows for an efficient, rapid, inexpensive and more objective protocol for the selection of recombinant baculovirus compared to the conventional protocol.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Goswami BB,Glazer RIsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
626-30issue
5eissn
0736-6205issn
1940-9818journal_volume
10pub_type
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