Abstract：
:The possibility to miniaturize and parallelize biological assays has a great impact on the development of biomedical technologies. Here, we describe a simple, miniaturized, and parallelized method employing entire cells from different cell lines displaying a protein of interest on their surface, which were immobilized on a microarray slide. Antibodies were added to these cellular microarrays, and their specific binding to the cell surface proteins was monitored using appropriate fluorescently labeled detection molecules. This new method is applicable for rapidly screening cell surface-specific antibodies with respect to selectivity and cross-reactivity.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Schwenk JM,Stoll D,Templin MF,Joos TOsubject
Has Abstractpub_date
2002-12-01 00:00:00pages
54-61eissn
0736-6205issn
1940-9818journal_volume
Supplpub_type
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