Abstract:
:Scanning conductance microscopy investigations were carried out in air on human chromosomes fixed on pre-fabricated SiO2 surfaces with a backgate. The point of the investigation was to estimate the dielectric constant of fixed human chromosomes in order to use it for microfluidic device optimization. The phase shift caused by the electrostatic forces, together with geometrical measurements of the atomic force microscopy (AFM) cantilever and the chromosomes were used to estimate a value for the dielectric constant of different human chromosomes.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Clausen CH,Lange JM,Jensen LB,Shah PJ,Dimaki MI,Svendsen WEdoi
10.2144/000112676subject
Has Abstractpub_date
2008-02-01 00:00:00pages
225-8issue
2eissn
0736-6205issn
1940-9818pii
000112676journal_volume
44pub_type
杂志文章相关文献
BIOTECHNIQUES文献大全abstract::RNA interference (RNAi) is a recent advance that provides the possibility to reduce the expression of specific target genes in cultured mammalian cells with potential applications on a genome-wide scale. However, to achieve this, robust methodologies that allow automated and efficient delivery of small interfering RNA...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/04373RT01
更新日期:2004-09-01 00:00:00
abstract::Changes in cardiac structure that depart from normal have generally been termed "remodeling". Assessment of ventricular remodeling at the cellular level should include measurement of myocyte dimensions. A well-established and reliable method to assess myocyte remodeling uses isolated cells and the Coulter Counter/Chan...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/98253pf02
更新日期:1998-09-01 00:00:00
abstract::Inverse PCR has been used for the recovery of genome regions flanking a known sequence, although its application to metagenome walking is limited due to inefficient amplification from low copy number fragments. Here we present an improved inverse PCR scheme that enables walking of rare fragments in environmental metag...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112210
更新日期:2006-08-01 00:00:00
abstract::Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure. In order to use DGS for large-scale genome structural studies, we have substantially revised the ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113294
更新日期:2009-11-01 00:00:00
abstract::To establish a nonradioactive method for demonstrating HPV DNA in routinely treated smears of the uterine cervix (alcohol fixation, staining according to Papanicolaou, preservation), in situ hybridizations were carried out in HeLa and SiHa cells grown on slides. After detailed investigations, the sensitivity and speci...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Microarray platforms require analytical pipelines with modules for data pre-processing including data normalization, statistical analysis for identification of differentially expressed genes, cluster analysis, and functional annotation. We previously developed the Automated Microarray Data Analysis (AMDA, version 2.3....
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/0000113889
更新日期:2012-07-01 00:00:00
abstract::Bovine serum albumin, free of deoxyribonuclease activity, was obtained in our laboratory using ion-exchange chromatography followed by acetylation. Chromatography on four different resins (DEAE-52, P-11, hydroxylapatite and Q Sepharose fast-flow) was examined. Fractions from Q Sepharose chromatography, eluted with a l...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::Real-time PCR is becoming a preferred method for quantification of minute amounts of nucleic acids. To achieve the full potential of this technique, accurate and convenient models for post-PCR data analysis are required. In this study, three different models were chosen to quantify the definitive copy numbers of Cucum...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112750
更新日期:2008-06-01 00:00:00
abstract::Gene expression analysis using high-density cDNA or oligonucleotide arrays is a rapidly emerging tool for transcriptomics, the analysis of the transcriptional state of a cell or organ. One of the limitations of current methodologies is the requirement of a relatively large amount of total or polyadenylated RNA as star...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/03343rr01
更新日期:2003-03-01 00:00:00
abstract::A simple spectrophotometric method for measuring DNA in proliferating cells is described. The method is an adaptation of the widely used diphenylamine (DPA) reaction to examine DNA in cells grown in a 96-well plate. This assay was capable of detecting as little as 10 ng DNA and could be used to measure DNA in stored a...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::New cloning vectors have been developed with features to enhance quick allelic exchange in gram-negative bacteria. The conditionally replicative R6K and transfer origins facilitate conjugation and chromosomal integration into a variety of bacterial species, whereas the sacB gene provides counterselection for allelic e...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0135
更新日期:2021-01-25 00:00:00
abstract::A pipeline has been created for the characterization of Arabidopsis thaliana mutants by generating flanking sequence tags (FSTs) and optimized for economic, high-throughput production. The GABI-Kat collection of T-DNA mutagenized A. thaliana plants was used as a source of independent transgenic lines. The pipeline inc...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/03356st01
更新日期:2003-12-01 00:00:00
abstract::The extraction of high-quality nucleic acid may be problematic in formalin-fixed tissues because of cross-linking between proteins and DNA. Old fixed tissue specimens do produce fragmented DNA (<1.2 kb), which is only used for PCR amplification. Here we show that high molecular weight DNA (>194 kb) can be successfully...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/02333rr03
更新日期:2002-09-01 00:00:00
abstract::Normalization is a critical step in the analysis of microarray gene expression data. For dual-labeled array, traditional normalization methods assume that the majority of genes are non-differentially expressed and that the number of overexpressed genes approximately equals the number of under-expressed genes. However,...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113195
更新日期:2009-08-01 00:00:00
abstract::Fluorescent live imaging of cells and embryos at subcellular resolution poses significant challenges for biologists due to morbidity and mortality ensuing from phototoxicity. Here we report the use of a spinning-disk confocal microscope to image mouse and bovine preimplantation embryos without impairing their developm...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112310
更新日期:2006-12-01 00:00:00
abstract::A dot immunoblotting technique has been developed to estimate the relative expression levels of tagged recombinant human proteins in mammalian cell culture media. Variations in sample denaturation, blocking agents and membrane composition and treatment were used to optimize the signal-to-noise ratio of the defined pro...
journal_title:BioTechniques
pub_type:
doi:10.2144/00285st07
更新日期:2000-05-01 00:00:00
abstract::Tandemly polymerized regulatory elements, antisense RNA segments or ribozymes are potentially useful in selective gene silencing. However, existing methods of tandemly polymerizing short DNA segments are laborious. We present a procedure that can create cloned arrays of 40-70 monomer units in two steps. We have create...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/00294st06
更新日期:2000-10-01 00:00:00
abstract::Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material. We describe a new technique, polymerase chain displacement reaction (PCDR), which uses multiple nested primers in...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113951
更新日期:2013-02-01 00:00:00
abstract::We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112960
更新日期:2008-11-01 00:00:00
abstract::We have altered the antibiotic resistance of the reporter plasmids and the pJG4-5 activation-domain and pEG202 DNA binding-domain plasmids used in the Brent interaction trap/two-hybrid system. These plasmids were each previously ampicillin-resistant, resulting in an inefficient purification of any one plasmid from a y...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/96212st02
更新日期:1996-08-01 00:00:00
abstract::Blue/white selection is the standard method for detecting a cloned DNA fragment. In the absence of an insert, uninterrupted expression of the vector-encoded alpha-complement of beta-galactosidase (beta-gal), results in the hydrolysis of X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galactoside) and the subsequent blue stai...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/01305pf01
更新日期:2001-05-01 00:00:00
abstract::We have compared RNA polymerase promoter activities in PCR-generated DNA fragments for use in the in vitro transcription of cRNA probes. Sense oligonucleotide primers, specific for the mouse acidic fibroblast growth factor gene, were synthesized with 5' extensions containing promoter sequences for the T7, T3 and SP6 R...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Cell culture is a vital component of laboratories throughout the scientific community, yet the absence of standardized protocols and documentation practice challenges laboratory efficiency and scientific reproducibility. We examined the effectiveness of a cloud-based software application, CultureTrax® as a tool for st...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0062
更新日期:2018-11-01 00:00:00
abstract::Identification of antimitotic molecules that affect tubulin dynamics is a multistep procedure. It includes in vitro tubulin polymerization assay, studies of a cell cycle effect, and general cytotoxicity assessment. To simplify this lengthy screening protocol, we have introduced and validated an assay system based on t...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112193
更新日期:2006-06-01 00:00:00
abstract::A simplified method for producing recombinant baculovirus for expression of foreign genes is described. The method utilizes insect cells infected with the wild-type virus before transfection with the plasmid transfer vector, instead of the standard procedure utilizing cotransfection with a plasmid and viral DNA. Recom...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide. ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/98252gt04
更新日期:1998-08-01 00:00:00
abstract::We have generated a genomic P1 bacteriophage library using Monterey pine (Pinus radiata) DNA. We first developed a method for isolating from pine tissue the very high molecular weight DNA necessary for the preparation of libraries requiring large inserts. The method involves protoplasting the cells, isolating nuclei a...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::To increase the reproducibility and to reduce the false positives in the initial mRNA differential display, modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods. Ten-base nucleotides were added at the 5' ends of the primers used in...
journal_title:BioTechniques
pub_type:
doi:
更新日期:1995-05-01 00:00:00
abstract::DNA-binding proteins can be purified by their affinity for probes with specific sequences that are tethered to magnetic beads. A restriction site at the end of the tether allows release of probe with factor still on it. This probe with bound factor can be bandshifted directly to compare to bandshifts in whole extracts...
journal_title:BioTechniques
pub_type:
doi:
更新日期:1994-05-01 00:00:00