Abstract:
:A dot immunoblotting technique has been developed to estimate the relative expression levels of tagged recombinant human proteins in mammalian cell culture media. Variations in sample denaturation, blocking agents and membrane composition and treatment were used to optimize the signal-to-noise ratio of the defined procedure. The method is rapid, with sensitivity extending to the low nanomolar range for a number of recombinant proteins. This technique should have general utility for antibody-based measurements of other tagged and non-tagged proteins in cell culture media or in biological fluids.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ellsworth JL,Hamacher N,Bagwell N,West JWdoi
10.2144/00285st07subject
Has Abstractpub_date
2000-05-01 00:00:00pages
934-6, 937issue
5eissn
0736-6205issn
1940-9818journal_volume
28pub_type
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