Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation.

Abstract:

:Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Barre FX,Mir LM,Lécluse Y,Harel-Bellan A

doi

10.2144/98252gt04

subject

Has Abstract

pub_date

1998-08-01 00:00:00

pages

294-6

issue

2

eissn

0736-6205

issn

1940-9818

journal_volume

25

pub_type

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