Abstract:
:Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Barre FX,Mir LM,Lécluse Y,Harel-Bellan Adoi
10.2144/98252gt04subject
Has Abstractpub_date
1998-08-01 00:00:00pages
294-6issue
2eissn
0736-6205issn
1940-9818journal_volume
25pub_type
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