Abstract:
:A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should stay in a complex with its mRNA and DNA. A method is designed to test these possibilities. Using an immobilized luciferase gene sequence, a functional luciferase protein is produced that remains associated through its mRNA with its DNA, confirming the feasibility of the proposed scheme. It has potential application for in vitro synthesis of proteins and protein micro-arrays.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Liu S,Shivashankar GV,Sano T,Libchaber Adoi
10.2144/00294st06subject
Has Abstractpub_date
2000-10-01 00:00:00pages
792, 794, 796, 798issue
4eissn
0736-6205issn
1940-9818journal_volume
29pub_type
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