Abstract:
:Quantitative real-time PCR has become a popular method to analyze and quantify changes in the copy number of mitochondrial DNA (mtDNA), and nuclear DNA (nDNA) is often used as an endogenous reference for mtDNA abundance. In our experience, using nDNA as a reference is problematic, due to differences in the extraction efficiency of nDNA and mtDNA and variation in the ploidy of experimental samples. Here, we report that the ratio of mtDNA to nDNA varies in repeated DNA extractions but that PhiX174 DNA, added before DNA extraction, is extracted with a similar efficiency to mtDNA, making it a suitable alternative reference for quantifying mtDNA copy number.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Myers MB,Mittelstaedt RA,Heflich RHdoi
10.2144/000113222subject
Has Abstractpub_date
2009-10-01 00:00:00pages
867-9issue
4eissn
0736-6205issn
1940-9818pii
000113222journal_volume
47pub_type
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