Abstract:
:Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Sharma D,Eichelberg MR,Haag JD,Meilahn AL,Muelbl MJ,Schell K,Smits BM,Gould MNdoi
10.2144/0000113854subject
Has Abstractpub_date
2012-07-01 00:00:00pages
57-60issue
1eissn
0736-6205issn
1940-9818pii
0000113854journal_volume
53pub_type
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