Abstract：
:Genotyping fish larvae is a valuable technique for numerous fields of study. While methods for collecting DNA from early stage larvae have been published, a non-lethal, non-invasive genotyping protocol for hatchlings that is amenable to high-throughput approaches is desirable. Here, we describe a method to individually genotype live, free-swimming, early fish larvae by characterizing their environmental DNA (eDNA). We demonstrate the utility of the method by assigning parentage to a sample (n = 50) of 3-5-day-old sheepshead minnow (Cyprinodon variegatus) larvae hatchlings, with very high rates of genotyping success (98%) and survival (92%) using mitochondrial and microsatellite DNA data. This method could be easily adapted to characterize early fish larvae from other model and non-model fish species, such as Danio rerio (zebrafish) and Medaka medaka.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Espinoza GJ,Poland JM,Bremer JRAdoi
10.2144/000114598subject
Has Abstractpub_date
2017-10-01 00:00:00pages
181-186issue
4eissn
0736-6205issn
1940-9818pii
000114598journal_volume
63pub_type
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