Abstract:
:In order to study quantitative gene expression with Northern blots, it is important to have an internal standard that can be used to verify even loading or to correct for uneven loading between lanes. In this study it is shown that two-dimensional quantitation of ethidium bromide-intercalated 28S rRNA fluorescence can be used for such standardization. It was found that the film response of the fluorescence was linear with respect to total loaded RNA in the range of 2.5-12.5 micrograms RNA under the conditions used, after which the linear relationship falls off. This method eliminates the use of radiation for internal standardization of Northern blots.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Bonini JA,Hofmann Csubject
Has Abstractpub_date
1991-12-01 00:00:00pages
708-710issue
6eissn
0736-6205issn
1940-9818journal_volume
11pub_type
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