Generation and characterization of recombinant vascular targeting agents from hybridoma cell lines.

Abstract:

:Vascular targeting agents (VTAs) can be produced by linking antibodies or antibody fragments directed against endothelial cell markers to effector moieties. So far, it has been necessary to produce the components of VTAs (antibody, antibody fragment, linker, and effector) separately and, subsequently, to conjugate them by biochemical reactions. We devised a cloning and expression system to allow rapid generation of recombinant VTAs from hybridoma cell lines. The VTAs consist of a single chain Fv antibody fragment as a targeting moiety and either truncated Pseudomonas exotoxin (resulting in immunotoxins) or truncated human tissue factor (resulting in coaguligands) as effectors. The system was applied to generate recombinant immunotoxins and coaguligands directed against endoglin, vascular endothelial growth factor (VEGF):VEGF receptor (VEGFR) complex and vascular cell adhesion molecule 1 (VCAM-1). The fusion proteins exhibited similar functional activity to analogous biochemical constructs. This is the first report to describe the generation and characterization of recombinant coaguligands.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Gottstein C,Wels W,Ober B,Thorpe PE

doi

10.2144/01301dd03

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

190-4, 196, 198 passim

issue

1

eissn

0736-6205

issn

1940-9818

journal_volume

30

pub_type

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