Abstract:
:Genome sequencing projects are either based on whole genome shotgun (WGS) or on a BAC-by-BAC strategy. Although WGS is in most cases the preferred choice, sometimes the BAC-by-BAC approach may be better because it requires a much simpler assembly process. Furthermore, when the study is limited to specific regions of the genome, the WGS would require an unjustified effort, making the BAC-by-BAC the only feasible strategy. In this paper we describe an informatics pipeline called PABS (Platform Assisted BAC-by-BAC Sequencing) that we developed to provide a tool to optimize the BAC-by-BAC sequencing strategy. PABS has two main functions: (i) PABS-Select, to choose suitable overlapping clones; and (ii) PABS-Validate, to verify whether a BAC under analysis is actually overlapping the neighboring BAC.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Todesco S,Campagna D,Levorin F,D'Angelo M,Schiavon R,Valle G,Vezzi Adoi
10.2144/000112686subject
Has Abstractpub_date
2008-01-01 00:00:00pages
60, 62, 64issue
1eissn
0736-6205issn
1940-9818pii
000112686journal_volume
44pub_type
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