Abstract:
:We describe a method using an inexpensive craft glue to routinely isolate specific areas of tissue as small as 1 mm2 from paraffin sections. The tissue may be digested to release nucleic acid suitable for PCR or reverse transcription PCR. The use of this procedure obviates the requirement for manual microdissection or ultraviolet light irradiation. Tissue remaining on the slide can be stained and analyzed, allowing the precision of the extraction to be determined. The slide can be stored as a permanent record of the material taken for analysis.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Turbett GR,Barnett TC,Dillon EK,Sellner LNdoi
10.2144/96205st04subject
Has Abstractpub_date
1996-05-01 00:00:00pages
846-50, 852-3issue
5eissn
0736-6205issn
1940-9818journal_volume
20pub_type
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