Abstract：
:The need for a primer hybridization step before sequencing has been eliminated using a stem-loop structure generated by PCR. The loop structure is obtained by careful design of the PCR primer or by cloning the target DNA into a dedicated vector (pRIT 28HP). After solid-phase capture of the PCR product, the loop is formed by elution of the non-bound strand. Here, we show that both the immobilized and the eluted strand can be analyzed using conventional Sanger DNA sequencing and the novel pyrosequencing method as described previously. By using a stem-loop structure as a primer for DNA sequencing, the risk for mispriming is minimized.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ronaghi M,Pettersson B,Uhlén M,Nyrén Pdoi
10.2144/98255rr02subject
Has Abstractpub_date
1998-11-01 00:00:00pages
876-8, 880-2, 884issue
5eissn
0736-6205issn
1940-9818journal_volume
25pub_type
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