Abstract:
:A procedure for amplification by PCR of reproducible allele markers for amplified fragment length polymorphism (Amp-FLP) analysis is presented. We have prepared markers for the allelic products of the VNTR loci D1S80 (MCT118) and D17S30 (YNZ22) and for the hypervariable VNTR locus close to the 3' end of the apolipoprotein B gene (apoB) by re-amplifying a mixture of PCR products from individuals with known alleles. These allele markers allow precise and discrete determination of the VNTR alleles at these loci using the Amp-FLP technique that should prove suitable in forensic analyses, paternity testing and population studies.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Sajantila A,Puomilahti S,Johnsson V,Ehnholm Csubject
Has Abstractpub_date
1992-01-01 00:00:00pages
16, 18, 20-2issue
1eissn
0736-6205issn
1940-9818journal_volume
12pub_type
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