Abstract：
:To move microarray technology into the diagnostic realm, the impact of technical parameters, such as sample preparation and RNA extraction, needs to be understood and minimized. We evaluated the impact of two RNA extraction methods, DNase treatment and the amount of hybridized cDNA probe, on the outcome of microarray results. The results for both RNA extraction methods were comparable, although one method resulted in residual DNA that slightly affected the microarray results. As little as one microgram of total RNA could be used to synthesize a cDNA probe and resulted in a gene expression profile that was similar to one produced using 5 micrograms total RNA, even though the overall signal intensity was lower. These experiments illustrate that microarray technology holds great promise for the use of limited clinical samples in the diagnostic setting.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ojaniemi H,Evengard B,Lee DR,Unger ER,Vernon SDdoi
10.2144/03355st04subject
Has Abstractpub_date
2003-11-01 00:00:00pages
968-73issue
5eissn
0736-6205issn
1940-9818journal_volume
35pub_type
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