Abstract：
:Specific amplification of nucleic acid sequences by PCR has been extensively used for the detection of gene rearrangements and gene expression. Although successful amplification of DNA sequences has been carried out with DNA prepared from formalin-fixed, paraffin-embedded (FFPE) tissues, there are only a few reports regarding RNA analysis in this kind of material. We describe a procedure for RNA extraction from different types of FFPE tissues, involving digestion with proteinase K followed by guanidinium-thiocyanate acid phenol extraction and DNase I digestion. These RNA preparations are suitable for PCR analysis of mRNA and even of intronless genes. Furthermore, the universally expressed porphobilinogen deaminase mRNA proved to be useful as a positive control because of the lack of pseudogenes.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Finke J,Fritzen R,Ternes P,Lange W,Dölken Gsubject
Has Abstractpub_date
1993-03-01 00:00:00pages
448-53issue
3eissn
0736-6205issn
1940-9818journal_volume
14pub_type
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