Abstract:
:A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step approach. In this work, we describe a gene cassette for the engineering of a conditional knock-down mutant, which allows the one-step targeted replacement of mycobacterial promoters by an anhydrotetracycline-inducible promoter. The functionality of this cassette was successfully tested by engineering conditional clpP and SecA1 mutants of Mycobacterium smegmatis.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Texier P,Coddeville M,Bordes P,Genevaux Pdoi
10.2144/btn-2018-0074subject
Has Abstractpub_date
2018-09-01 00:00:00pages
159-162issue
3eissn
0736-6205issn
1940-9818journal_volume
65pub_type
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