Abstract:
:Advances in in vitro display and protein engineering yield therapeutics with affinities in the picomolar range. The Gyrolab® microfluidics platform uses the kinetic exclusion assay principle to measure subnanomolar solution affinities. This work describes application of the Gyrolab solution affinity module and the new multi-curve analysis feature to determine affinity of the PD-L1 Adnectin™ positron emission tomography radioligand, which was measured as 20 pM for human PD-L1. We also report key parameters that affect assay signal-to-background ratio and data quality, such as detection reagent concentration. Gyrolab offers the necessary throughput for rapid assay development with low sample consumption, as demonstrated in this study, which also provides helpful tips for assay optimization for solution affinity measurement.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Dai Z,Juneja J,Schneeweis L,Cohen D,Marsilio F,Morin P,DasGupta Rdoi
10.2144/btn-2020-0080subject
Has Abstractpub_date
2020-09-01 00:00:00pages
200-205issue
3eissn
0736-6205issn
1940-9818journal_volume
69pub_type
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