Abstract:
:The technique described here is a fast and simple method of extracting chloroplast DNA (cpDNA). It overcomes the need for differential centrifugation using density gradients. The leaves do not have to be kept in the dark and lyophilized before extraction, but lyophilization is still possible. The chloroplasts are specifically lysed in a cell extract of leaves, using a non-ionic detergent. After isolation by centrifugation, the cpDNA is purified by the combined action of proteolytic enzymes and detergents, followed by the elimination of proteins using a mixture of chloroform and isoamyl alcohol. This method provided good quality restriction profiles for all species analyzed.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Mariac C,Trouslot P,Poteaux C,Bezançon G,Renno JFdoi
10.2144/00281st07subject
Has Abstractpub_date
2000-01-01 00:00:00pages
110-3issue
1eissn
0736-6205issn
1940-9818journal_volume
28pub_type
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