Abstract:
:The analysis of DNA restriction fragment length polymorphisms by Southern blot hybridization requires that sufficient quantities of high molecular weight genomic DNA be extracted from biological specimens. Prior to analysis, it is necessary to determine the quantity and quality of the extracted DNA. For many applications, it is also desirable to determine the amount of DNA which is of human origin. In this report, we describe a simple and highly sensitive procedure for the specific quantification of human genomic DNA in forensic extracts or any biological sample. A small fraction of the extract is immobilized onto a nylon membrane and subsequently hybridized to p17H8 (D17Z1), a cloned probe which detects highly repetitive, primate-specific alpha satellite DNA. The procedure requires less than four hours to complete and can be used to quantify subnanogram amounts of hybridizable human genomic DNA.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Waye JS,Presley LA,Budowle B,Shutler GG,Fourney RMsubject
Has Abstractpub_date
1989-09-01 00:00:00pages
852-5issue
8eissn
0736-6205issn
1940-9818journal_volume
7pub_type
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