Abstract:
:The relative spatial distribution of cells in a solid tumor contributes to development of malignancy, yet the details of this process remain poorly understood. To elucidate these mechanisms, the ability to extract and analyze the entire DNA content of individual cells whose precise location in the tumor is known is required, yet such methodology has not yet been described. Here we detail a procedure to directly extract complete individual nuclei from fixed-frozen tissue sections using through-focus analysis coupled with laser microdissection, followed by whole genome amplification. We show that this technique is suitable for routine evaluation of genomic variation such as SNP analyses of the specifically selected nuclei. Our method should provide a means for whole genome variation studies of single cells from spatially defined positions within tumor tissues.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Guo Y,Yang Y,Zhou J,Czajkowsky DM,Liu B,Shao Zdoi
10.2144/000113860subject
Has Abstractpub_date
2012-04-01 00:00:00issue
4eissn
0736-6205issn
1940-9818pii
000113860journal_volume
52pub_type
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