Abstract:
:Pairs of primers flanking known miniTn10 transposon insertion sites were used to confirm the presence of the transposon in DNA isolated from Legionella pneumophila mutants. It was expected that the polymerase chain reaction products derived from the mutant template would be larger than those from the wild-type (WT) template due to the presence of the 1.8-kb transposon. Instead, it was observed that the mutant template yielded a product of almost the same size as that yielded by WT template. We present evidence to indicate that the aberrant product from the mutant template is a direct result of secondary structure of the template resulting from an inverted repeat sequence present in the miniTn10 transposon.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Viswanathan VK,Krcmarik K,Cianciotto NPdoi
10.2144/99273st04subject
Has Abstractpub_date
1999-09-01 00:00:00pages
508-11issue
3eissn
0736-6205issn
1940-9818journal_volume
27pub_type
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