Abstract：
:Millions of museum specimens are integral to biodiversity studies; however, DNA degradation may limit the ability to obtain DNA sequences. In this study, a degradation analysis model for Lepidoptera specimens was established. Based on this model, we revealed the characteristics of DNA fragment distribution caused by external DNA damage factors during specimen preservation. We found that the degree of DNA degradation increased over time; DNA degradation of spread and dried adult specimens was significantly higher than that in the folded and formalin-fixed larval specimens. However, the effects of folding wings on DNA degradation and the effects of the preservation method/stage (formalin-fixed larval vs air-dried adult specimens) were different for different species.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Xu Y,Ren X,Wang H,Wang M,Li Gdoi
10.2144/btn-2019-0166subject
Has Abstractpub_date
2020-03-01 00:00:00pages
138-147issue
3eissn
0736-6205issn
1940-9818journal_volume
68pub_type
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