Abstract:
:Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes-SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, EvaGreen-in three different qLAMP model systems. SYTO-9 and SYTO-82, which had the best results, were used for additional enzyme and template titration studies. SYTO-82 demonstrated the best combination of time-to-threshold (Tt) and signal-to-noise ratio (SNR).
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Oscorbin IP,Belousova EA,Zakabunin AI,Boyarskikh UA,Filipenko MLdoi
10.2144/000114432subject
Has Abstractpub_date
2016-07-01 00:00:00pages
20-5issue
1eissn
0736-6205issn
1940-9818pii
000114432journal_volume
61pub_type
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