Abstract:
:A fluorescent in situ DNA hybridization assay employing a biotinylated DNA probe was used to visualize single copies of human immunodeficiency virus (HIV) proviral DNA in the nuclei and metaphase chromosomes of infected cells. In clonal cell lines that contain either one or two copies of proviral DNA, the efficiency of detection of individual loci of proviral DNA was 57% to 78%. Only 1% of uninfected control cells exhibited a false-positive signal. HIV proviral DNA could be accurately identified in mixed populations comprised of only 5% infected cells. Thus, this assay could be used to identify cells that harbor HIV proviral DNA and to monitor the status of proviral DNA throughout the course of HIV infection.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Spadoro JP,Payne H,Lee Y,Rosenstraus MJsubject
Has Abstractpub_date
1990-08-01 00:00:00pages
186-95issue
2eissn
0736-6205issn
1940-9818journal_volume
9pub_type
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