Simultaneous use of standard and low-melting agarose for the separation and isolation of DNA by electrophoresis.

Abstract:

:In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential digestion and isolation, characteristic of low-melting agarose.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Fotadar U,Shapiro LE,Surks MI

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

171-2

issue

2

eissn

0736-6205

issn

1940-9818

journal_volume

10

pub_type

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