Abstract:
:This report describes a common method of obtaining template DNA from phagemids, phages and plasmids. The strategy is based on the use of the cationic detergent cetyl-trimethylammonium bromide (CTAB) for DNA precipitation. By avoiding phase separation, many manipulation steps are reduced. A time-saving modification to perform double-stranded DNA sequencing directly after alkaline-denaturation is also introduced. The protocols described here allow the researcher to obtain template DNA from a variety of initial sources, thus giving reproducible sequencing results when using T7 DNA polymerase.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Del Sal G,Manfioletti G,Schneider Csubject
Has Abstractpub_date
1989-05-01 00:00:00pages
514-20issue
5eissn
0736-6205issn
1940-9818journal_volume
7pub_type
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