Abstract:
:To gain insightful information about the mechanisms through which genes are activated and repressed requires gene reporter systems that are sensitive, robust, and cost-effective. Although numerous reporter gene technologies are commercially available, none are as sophisticated and user-friendly as beta-lactamase (BLA) when it comes to studying gene expression in live cells. This article presents an overview of the BLA technology and describes how it can be exploited for studying rare events such as homologous recombination in somatic cells and be used to deliver any DNA sequence of choice anywhere within the genome.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Qureshi SAdoi
10.2144/000112292subject
Has Abstractpub_date
2007-01-01 00:00:00pages
91-6issue
1eissn
0736-6205issn
1940-9818pii
000112292journal_volume
42pub_type
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