Abstract:
:We report a simple and inexpensive method to quantitate loading and transfer of RNA and to examine RNA integrity for use with Northern hybridization. This technique involves quantitation by computer-assisted video densitometry of a negative photograph of 28S and 18S rRNA from ethidium bromide-stained RNA fixed to nylon membranes. This method eliminates the issue of variability of expression of "housekeeping" genes and the need for a second round of hybridization to quantitate control probes.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Correa-Rotter R,Mariash CN,Rosenberg MEsubject
Has Abstractpub_date
1992-02-01 00:00:00pages
154-8issue
2eissn
0736-6205issn
1940-9818journal_volume
12pub_type
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