Abstract:
:Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays for measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) and TK1 ELISA from Abcam. Overall, the TK 210 ELISA showed higher sensitivity than the Abcam TK1 ELISA for differentiating hematological malignancies (sensitivity of 0.77 vs 0.45) as well as for distinguishing sera of patients with solid tumors from those of apparently healthy individuals (0.61 vs 0.20). There was no significant difference in the TK1 protein levels determined with the TK 210 ELISA between different age groups from apparently healthy individuals. These results strongly indicate that the AroCell TK 210 ELISA is accurate and sensitive enough to be a valuable tool in cancer management.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Kumar JK,Holmgren S,Levedahl KH,Höglund M,Venge P,Eriksson Sdoi
10.2144/btn-2019-0148subject
Has Abstractpub_date
2020-06-01 00:00:00pages
334-341issue
6eissn
0736-6205issn
1940-9818journal_volume
68pub_type
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