Abstract：
:The study of gene regulation in cardiac myocytes requires a reliable in vitro model. However, monolayer cultures used for this purpose are typically not exposed to electrical stimulation, though this has been shown to strongly affect cardiomyocyte gene expression. Based on pacemakers for clinical use, we developed an easy-to-use portable system that allows the user to perform electro-stimulation of cardiomyocyte cultures in standard tissue incubators without the need for bulky equipment. In addition, we present a refined protocol for culturing high-purity cardiomyocyte cultures with excellent contractile properties for a wide variety of applications.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Martherus RS,Zeijlemaker VA,Ayoubi TAdoi
10.2144/000113308subject
Has Abstractpub_date
2010-01-01 00:00:00pages
65-7issue
1eissn
0736-6205issn
1940-9818pii
000113308journal_volume
48pub_type
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