Abstract：
:We report a modification of a liquid hybridization method that serves as a rapid, sensitive alternative to Southern hybridization for the analysis of polymerase chain reaction (PCR) products. An aliquot of the completed PCR is mixed with an internally nested, end-labeled oligonucleotide probe, and one cycle of PCR is performed. The products are electrophoresed, dried and analyzed by autoradiography. The method is significantly more sensitive than standard Southern hybridization methods, is equally specific, requires as little as 1-10 picograms of probe and results can be obtained in less than an hour. The procedure is equally useful for identifying products in multiplex PCRs or single-stranded PCRs.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Parker JD,Burmer GCsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
94-101issue
1eissn
0736-6205issn
1940-9818journal_volume
10pub_type
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