Abstract：
:Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression. However, the expression of more than one gene cannot be monitored simultaneously by this system because one of the two reporting luciferases must be used as an internal control. We have developed a novel reporter assay system in which three luciferases that emit green, orange, and red light with a single substrate are used as reporter genes. The activities of the luciferases can be measured simultaneously and quantitatively with optical filters. This system enables us to simply and rapidly monitor multiple gene expressions in a one-step reaction.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Nakajima Y,Kimura T,Sugata K,Enomoto T,Asakawa A,Kubota H,Ikeda M,Ohmiya Ydoi
10.2144/05386ST03subject
Has Abstractpub_date
2005-06-01 00:00:00pages
891-4issue
6eissn
0736-6205issn
1940-9818pii
05386ST03journal_volume
38pub_type
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