Abstract：
:The use of AlphaScreen® detection has allowed researchers to examine a wide variety of molecular interactions for use in high-throughput screening. However, the cost of Alpha reagents can often be prohibitory for extended screening campaigns or for young investigators with limited funding. To reduce assay costs, many labs have focused on miniaturization, while there have been limited efforts to scale down Alpha reagents. Thus, we describe the optimization of an AlphaScreen detection platform by systematically reducing the Alpha reagents down to 2.5 μg/ml beads, without compromising assay integrity. We demonstrate that reducing bead concentration reduces detection costs substantially while yielding robust statistics. We believe this simple new protocol will enhance the future utilization of AlphaScreen technology in high-throughput screening.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Veloria JR,Devkota AK,Cho EJ,Dalby KNdoi
10.2144/btn-2018-2001subject
Has Abstractpub_date
2018-04-01 00:00:00pages
181-183issue
4eissn
0736-6205issn
1940-9818journal_volume
64pub_type
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