Abstract:
:The concentration of proteins in cells is an important parameter that determines how a protein will interact with other proteins or pharmacological agents. Recent developments in Western blotting techniques have now made this a method of choice to measure protein concentration in complex solutions such as total cell extracts. We show that detection of Cy5-coupled secondary antibodies by PhosphorImager analysis produces signals that approach linearity with respect to protein concentration over a 20-fold range. We used this technique to estimate cellular levels of zyxin, which is an important protein component of the actin cytoskeleton in mammalian cells. By producing specific protein standards based on sequences that are available from public databases, it is now possible to estimate the concentration of almost any protein by this technique.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Fradelizi J,Friederich E,Beckerle MC,Golsteyn RMsubject
Has Abstractpub_date
1999-03-01 00:00:00pages
484-6, 488, 490 passimissue
3eissn
0736-6205issn
1940-9818journal_volume
26pub_type
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