Abstract:
:Expanding applications of cDNA microarrays such as fine needle aspiration biopsy and laser capture microdissection necessitate the ability to perform arrays with minute starting amounts of RNA. While methods for amplifying RNA have been advocated, the fidelity of array results using amplified material has not been fully validated. Here we demonstrate preserved fidelity in arrays using one or two rounds of mRNA amplification, validated by downstream real-time quantitative PCR. In addition, the quality of the array data was superior to that obtained using total RNA. Based on these results, we recommend routine mRNA amplification for all cDNA microarray-based analysis of gene expression.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Feldman AL,Costouros NG,Wang E,Qian M,Marincola FM,Alexander HR,Libutti SKdoi
10.2144/02334mt04subject
Has Abstractpub_date
2002-10-01 00:00:00pages
906-12, 914issue
4eissn
0736-6205issn
1940-9818journal_volume
33pub_type
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