Abstract：
:Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene synthesis or decrease variability when applied to the construction of randomized libraries. We have developed a simple DNA polymerase-based gene synthesis reaction, polymerase step reaction (PSR), that assembles DNA oligonucleotides in a unidirectional fashion without the need for amplification. We demonstrate that PSR is efficient, with little off-product production, no detectable error propagation, and maximized variability in the synthesis of a phage display library.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Lee ZB,Firnhaber C,Clarke J,DeDecker BSdoi
10.2144/000114330subject
Has Abstractpub_date
2015-09-01 00:00:00pages
163-6issue
3eissn
0736-6205issn
1940-9818pii
000114330journal_volume
59pub_type
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