:At many research institutions, lab space is more valuable than gold. Developers are taking note by designing smaller instruments with enhanced capabilities. Nathan Blow looks inside today's tiny lab.






Blow NS Ph D




Has Abstract


2017-01-01 00:00:00














  • Genetic immunization for antibody generation in research animals by intravenous delivery of plasmid DNA.

    abstract::Genetic immunization is an attractive approach to generate antibodies because native proteins are expressed in vivo with normal posttranscriptional modifications, avoiding time-consuming and costly antigen isolation or synthesis. Hydrodynamic tail or limb vein delivery of naked plasmid DNA expression vectors was used ...


    pub_type: 杂志文章


    authors: Bates MK,Zhang G,Sebestyén MG,Neal ZC,Wolff JA,Herweijer H

    更新日期:2006-02-01 00:00:00

  • Simultaneous mutagenesis of multiple sites: application of the ligase chain reaction using PCR products instead of oligonucleotides.

    abstract::A method is described for preparing mutants with multiple, site-directed mutations by ordered coupling of PCR-generated fragments catalyzed by a thermostable DNA ligase. Annealing of the sense strands of the fragments to a single-stranded (antisense) template created a full-length sense strand leaving only nicks that ...


    pub_type: 杂志文章


    authors: Rouwendal GJ,Wolbert EJ,Zwiers LH,Springer J

    更新日期:1993-07-01 00:00:00

  • Use of CMFDA and CMTMR fluorescent dyes in FACS-based antibody screening.

    abstract::Cell-based immunizations are often used when membrane antigens are difficult to purify. To confirm that an antibody binding to the surface of a cell line is, in fact, binding to the desired antigen, FACS can be performed independently on two cell lines, a transfected cell line expressing the antigen of interest and a ...




    authors: Yang XP,Gallo M,Ngan I,Nocerini M,Chen MM

    更新日期:2002-03-01 00:00:00

  • Scoring insertion-deletion polymorphisms by dynamic allele-specific hybridization.

    abstract::Genome variation provides researchers with thousands of markers with which to study human demographic history and phenotypes. Insertion-deletion (indel) polymorphism is an important and abundant form of human genome variation, and convenient methods for genotyping indels are therefore needed. Here we evaluate dynamic ...


    pub_type: 杂志文章


    authors: Sawyer SL,Howell WM,Brookes AJ

    更新日期:2003-08-01 00:00:00

  • Delivery of protein into cells using polycationic liposomes.

    abstract::We describe a procedure for delivery of purified proteins into a variety of tissue culture cells using a new polycationic lipid preparation, LipofectAMINE. Several different proteins, with diverse physical properties, can be delivered into cells by this method. Compared with commercially available monocationic lipids,...




    authors: Sells MA,Li J,Chernoff J

    更新日期:1995-07-01 00:00:00

  • Pulse-first heterofusion of cells by electric field pulses and associated loading of macromolecules into mammalian cells.

    abstract::Exposing cells to brief, high-intensity electrical field pulses can lead to the permeabilization of their plasma membranes. This electro-induced permeated state of the cell membrane is reversible and leads to cell fusion; i.e., the electropermeabilized state if fusogenic. The size of cells intended for fusing, however...


    pub_type: 杂志文章


    authors: Rols MP,Dahhou F,Teissié J

    更新日期:1994-10-01 00:00:00

  • BLM Analyzer: a software tool for experiments on planar lipid bilayers.

    abstract::Planar lipid bilayers represent a versatile platform for studying the functions of various membrane proteins as well as the development of biosensors. Despite the continuing technological progress in the fabrication of low-noise bilayer setups with mechanically and electrically stable planar bilayers, there is still a...


    pub_type: 杂志文章


    authors: Novák P,Gaburjáková M,Zahradník I

    更新日期:2007-03-01 00:00:00

  • FoLT PCR: a simple PCR protocol for amplifying DNA directly from whole blood.

    abstract::FoLT (formamide low temperature) PCR is a protocol for amplifying DNA directly from whole blood without any preparative steps. Up to 10% (vol/vol) whole blood can be added directly into the tube containing the PCR mixture. There is no need for transfers, centrifugations, pre-boiling or any preparative step. It involve...


    pub_type: 杂志文章


    authors: Panaccio M,Georgesz M,Lew AM

    更新日期:1993-02-01 00:00:00

  • Limitations for purification of murine interleukin-18 when expressed as a fusion protein containing the FLAG peptide.

    abstract::As a strategy to purify recombinant murine Interleukin (IL)-18, we cloned the mature coding region of this protein into the pFLAG-1 expression system. The intent was to use the FLAG peptide "tag" as an amino terminal addition to IL-18 so that purification of this fusion protein (FLAG-IL-18) on anti-FLAG antibody affin...




    authors: Elhofy A,Bost KL

    更新日期:1998-09-01 00:00:00

  • DNAOPT: a computer program to aid optimization of DNA gel electrophoresis and SDS-PAGE.

    abstract::Several methods and computer programs have been developed for estimating the size of DNA fragments from gel electrophoresis. However, methods are lacking that may facilitate in optimization of gel conditions. In this article, a computer program called DNAOPT is described, which was developed to assist researchers in t...




    authors: Raghava GP

    更新日期:1995-02-01 00:00:00

  • An improved Huffman coding method for archiving text, images, and music characters in DNA.

    abstract::An improved Huffman coding method for information storage in DNA is described. The method entails the utilization of modified unambiguous base assignment that enables efficient coding of characters. A plasmid-based library with efficient and reliable information retrieval and assembly with uniquely designed primers is...


    pub_type: 杂志文章


    authors: Ailenberg M,Rotstein O

    更新日期:2009-09-01 00:00:00

  • Formation of tight monolayers of guinea pig airway epithelial cells cultured in an air-interface: bioelectric properties.

    abstract::In this study, we have developed an air-interface culture system in which guinea pig tracheobronchial epithelial (GPTE) cells rapidly form tight monolayers. Enzymatically isolated GPTE cells were plated on collagen-treated polycarbonate microporous cell culture inserts at a density of 10(6) cells/cm2 (day 0). Bioelect...


    pub_type: 杂志文章


    authors: Robison TW,Dorio RJ,Kim KJ

    更新日期:1993-09-01 00:00:00

  • CRISPR vs COVID-19: how can gene editing help beat a virus?

    abstract::[Formula: see text] Known to be a sturdy weapon in a scientist's arsenal, how has the gene editing tool CRISPR been applied in the fight against COVID-19? ...


    pub_type: 新闻


    authors: Straiton J

    更新日期:2020-11-01 00:00:00

  • Antibody-mediated soluble CD14 stabilization prevents agitation-induced increases in presepsin levels in blood component specimens.

    abstract::Presepsin is a 13-kDa N-terminal glycoprotein of CD14. Previously, agitation-induced increases in presepsin levels have been reported; however, the mechanism remains poorly understood. In this study, we aimed to reveal the mechanism of presepsin increase. The agitated plasma or serum was separated using gel exclusion ...


    pub_type: 杂志文章


    authors: Morino G,Takahashi G,Kan S,Inoue Y,Sato K,Shirakawa K

    更新日期:2021-01-29 00:00:00

  • QSYP peptide sequence is selected from phage display libraries by bovine IgG contaminants in monoclonal antibody preparations.

    abstract::A consensus peptide sequence, QSYP, appears as an artifact during the mapping of monoclonal antibodies (MAbs) using a random peptide phage display library. Phage bearing this QSYP sequence were independently selected by four different laboratories screening separate MAb preparations with the same phage library. In eac...


    pub_type: 杂志文章


    authors: Jacobs JM,Bailey BW,Burritt JB,Morrison SG,Morrison RP,Dratz EA,Jesaitis AJ,Teintze M

    更新日期:2003-01-01 00:00:00

  • Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources.

    abstract::A modification of the TRI Reagent procedure has been elaborated for isolation of RNA from polysaccharide- and proteoglycan-rich material. In the modified procedure, RNA is precipitated from the aqueous phase by the combined action of isopropanol and a high-salt concentration. Under these conditions, RNA is effectively...


    pub_type: 杂志文章


    authors: Chomczynski P,Mackey K

    更新日期:1995-12-01 00:00:00

  • En masse analysis of nascent translation using microarrays.

    abstract::We report a robust method for studying en masse changes in translation using cDNA arrays. The relative distribution of messenger RNAs (mRNAs) along polysome gradients was monitored by performing cDNA array analysis of each gradient fraction and quantifying the mRNA translational status by regression analysis. Using th...


    pub_type: 杂志文章


    authors: Mazan-Mamczarz K,Kawai T,Martindale JL,Gorospe M

    更新日期:2005-07-01 00:00:00

  • Rapid colchicine competition-binding scintillation proximity assay using biotin-labeled tubulin.

    abstract::We have developed a rapid [3H]colchicine competition-binding scintillation proximity assay (SPA) to evaluate antimitotic compounds that bind to the colchicine-binding site on tubulin. The premise of our assay is that compounds will compete with radiolabeled colchicine for the tubulin-binding domain. Biotin-labeled tub...


    pub_type: 杂志文章


    authors: Tahir SK,Kovar P,Rosenberg SH,Ng SC

    更新日期:2000-07-01 00:00:00

  • An electrophoretic capture method for efficient recovery of rare sequences from heterogeneous DNA.

    abstract::It is difficult to isolate rare, PCR-quality DNA from specimens containing large quantities of nonspecific DNA from multiple sources (heterogeneous DNA). Extracting human DNA from stool for colorectal cancer (CRC) screening tests exemplifies this technically challenging sample preparation problem. The stool matrix is ...


    pub_type: 杂志文章


    authors: Kent Moore J,Smith JA,Whitney DH,Durkee KH,Shuber AP

    更新日期:2008-03-01 00:00:00

  • Effective flow cytometric phenotyping of cells using minimal amounts of antibody.

    abstract::Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate resul...


    pub_type: 杂志文章


    authors: Sharma D,Eichelberg MR,Haag JD,Meilahn AL,Muelbl MJ,Schell K,Smits BM,Gould MN

    更新日期:2012-07-01 00:00:00

  • Template secondary structure promotes polymerase jumping during PCR amplification.

    abstract::Pairs of primers flanking known miniTn10 transposon insertion sites were used to confirm the presence of the transposon in DNA isolated from Legionella pneumophila mutants. It was expected that the polymerase chain reaction products derived from the mutant template would be larger than those from the wild-type (WT) te...




    authors: Viswanathan VK,Krcmarik K,Cianciotto NP

    更新日期:1999-09-01 00:00:00

  • Aptamer-dependent full-length cDNA synthesis by overlap extension PCR.

    abstract::Sequencing of the human genome in combination with computational annotation has provided tremendous data on predicted genes. However, for most of them, no corresponding cDNAs are available yet. Furthermore, even where cDNA clones were obtained, gene transcripts often have many different splice variants that are not co...


    pub_type: 杂志文章


    authors: Mitani Y,Nakayama T,Harbers M,Hayashizaki Y

    更新日期:2004-07-01 00:00:00

  • Efficiency of transduction by recombinant Sindbis replicon virus varies among cell lines, including mosquito cells and rat sensory neurons.

    abstract::Recombinant alphaviruses have been used as vehicles for delivery and expression of heterologous genes in mammalian, avian and insect cell lines. We have used a Sindbis replicon virus (Sinreplac) able to express the E. coli lacZ gene to compare the efficiency of transduction in one insect, six mammalian cell lines and ...


    pub_type: 杂志文章


    authors: Corsini J,Traul DL,Wilcox CL,Gaines P,Carlson JO

    更新日期:1996-09-01 00:00:00

  • In situ hybridization with digoxigenin-labeled DNA of human papillomaviruses (HPV 16/18) in HeLa and SiHa cells.

    abstract::To establish a nonradioactive method for demonstrating HPV DNA in routinely treated smears of the uterine cervix (alcohol fixation, staining according to Papanicolaou, preservation), in situ hybridizations were carried out in HeLa and SiHa cells grown on slides. After detailed investigations, the sensitivity and speci...


    pub_type: 杂志文章


    authors: Heiles HB,Genersch E,Kessler C,Neumann R,Eggers HJ

    更新日期:1988-11-01 00:00:00

  • Simultaneous use of standard and low-melting agarose for the separation and isolation of DNA by electrophoresis.

    abstract::In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential diges...


    pub_type: 杂志文章


    authors: Fotadar U,Shapiro LE,Surks MI

    更新日期:1991-02-01 00:00:00

  • Assessment of three-dimensional biofilm structure using an optical microscope.

    abstract::A method allowing the evaluation of the structure and the calculation of the volume of a biofilm, using an optical microscope, is proposed based on the linear relation between the intensity of a pixel in biofilm images grabbed on the x-y plane and the corresponding number of cells in the z direction, which allows the ...


    pub_type: 杂志文章


    authors: de Carvalho CC,da Fonseca MM

    更新日期:2007-05-01 00:00:00

  • AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays.

    abstract::Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays for measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) and TK1 ELISA from Abcam. Overall, the ...


    pub_type: 杂志文章


    authors: Kumar JK,Holmgren S,Levedahl KH,Höglund M,Venge P,Eriksson S

    更新日期:2020-06-01 00:00:00

  • High-performance subtractive hybridization of cDNAs by covalent bonding between specific complementary nucleotides.

    abstract::We have developed an improved subtractive hybridization method that provides a fast, simple and reliable isolation of desired different sequences from two compared DNA libraries, one of which contains all unwanted homologues (subtracter) and another contains certain desired heterologues (tester). The DNA library can b...


    pub_type: 杂志文章


    authors: Ying SY,Lin S

    更新日期:1999-05-01 00:00:00

  • Single copies of HIV proviral DNA detected by fluorescent in situ hybridization.

    abstract::A fluorescent in situ DNA hybridization assay employing a biotinylated DNA probe was used to visualize single copies of human immunodeficiency virus (HIV) proviral DNA in the nuclei and metaphase chromosomes of infected cells. In clonal cell lines that contain either one or two copies of proviral DNA, the efficiency o...


    pub_type: 杂志文章


    authors: Spadoro JP,Payne H,Lee Y,Rosenstraus MJ

    更新日期:1990-08-01 00:00:00

  • Determination of human NAT2 acetylator genotype by oligonucleotide ligation assay.

    abstract::The oligonucleotide ligation assay (OLA) was adapted to the genotyping of the N-arylamine-acetyltransferase (NAT2) gene. This assay allows the use of 96-well microplates and robotic workstations for high sample throughput. We found this assay to be accurate, efficient and reliable. Another advantage for epidemiologica...


    pub_type: 杂志文章


    authors: Bigler J,Chen C,Potter JD

    更新日期:1997-04-01 00:00:00