Abstract：
:Three-dimensional collagen gel contraction is the standard assay utilized for functionally quantifying a variety of cell types, in particular smooth muscle cells (SMCs) and myofibroblasts. Here, we have developed a method to effectively reduce the three-dimensional parameters of the standard collagen gel into a single, linear measurement. Cell/collagen suspensions that are cast into glass capillary tubes provide several advantages over the well plate format, such as eliminating the need for digital imaging equipment and software to quantify the amount of cellular contraction. In addition, capillary tube gels require significantly fewer cells and far less reagents than standard methods.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ilagan R,Guthrie K,Quinlan S,Rapoport HS,Jones S,Church A,Basu J,Ludlow Jdoi
10.2144/000113349subject
Has Abstractpub_date
2010-02-01 00:00:00pages
153-5issue
2eissn
0736-6205issn
1940-9818pii
000113349journal_volume
48pub_type
杂志文章相关文献
BIOTECHNIQUES文献大全abstract：:A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and ho...
journal_title：BioTechniques
pub_type：
doi：10.2144/00291st01
更新日期：2000-07-01 00:00:00
abstract：:We describe a simple and efficient technique that facilitates the amplification of specific mRNA for cloning and sequencing purposes. An mRNA bound to a small piece of membrane filter is used as a template to synthesize complementary DNA. The product of this reaction is then transferred to a new tube and amplified usi...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1993-11-01 00:00:00
abstract：:Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection. Our results demonstrate that coupling egg wh...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000113914
更新日期：2012-08-01 00:00:00
abstract：:A method is described for preparing mutants with multiple, site-directed mutations by ordered coupling of PCR-generated fragments catalyzed by a thermostable DNA ligase. Annealing of the sense strands of the fragments to a single-stranded (antisense) template created a full-length sense strand leaving only nicks that ...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:The concentration of proteins in cells is an important parameter that determines how a protein will interact with other proteins or pharmacological agents. Recent developments in Western blotting techniques have now made this a method of choice to measure protein concentration in complex solutions such as total cell e...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1999-03-01 00:00:00
abstract：:We have generated a genomic P1 bacteriophage library using Monterey pine (Pinus radiata) DNA. We first developed a method for isolating from pine tissue the very high molecular weight DNA necessary for the preparation of libraries requiring large inserts. The method involves protoplasting the cells, isolating nuclei a...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:Enhanced green fluorescent protein (EGFP) is the preferred reporter protein for real-time detection in individual cells, but its usefulness for gene expression quantification is limited by the sensitivity of standard detection techniques. We tested whether the unique feature of single-cell detection and quantification...
journal_title：BioTechniques
pub_type：
doi：10.2144/02321st01
更新日期：2002-01-01 00:00:00
abstract：:One of the challenges of performing live-cell imaging in plants is establishing a system for securing the sample during imaging that allows for the rapid addition of treatments. Here we report how a commercially available device called a HybriWell™ can be repurposed to create an imaging chamber suitable for Arabidopsi...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/btn-2018-0044
更新日期：2018-06-01 00:00:00
abstract：:A fully automated nucleic acid analysis system is described, which offers positive sample identification, improved sensitivity and reduced user interaction compared to conventional techniques. The system relies on the sequence-specific capture of DNA onto solid-phase particles, confirming product identity without the ...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/99271pf01
更新日期：1999-07-01 00:00:00
abstract：:Microsatellite sequences are important markers for population genetics studies. In the past, the development of adequate microsatellite primers has been cumbersome. However with the advent of next-generation sequencing technologies, marker identification in genomes of non-model species has been greatly simplified. Her...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000114104
更新日期：2013-11-01 00:00:00
abstract：:A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should ...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/00294st06
更新日期：2000-10-01 00:00:00
abstract：:A nonimmune phagemid recombinant antibody fragment (rFab) library was generated with a nominal diversity of 1.16 x 10(7) using the QuikChange Multi Site-Directed Mutagenesis kit. Two degenerate primers spanning the third complementarity-determining region (CDR) loops of the antibody fragment light and heavy chain were...
journal_title：BioTechniques
pub_type：
doi：10.2144/03354st05
更新日期：2003-10-01 00:00:00
abstract：:A technique that can be used to isolate vector/insert junctions from clones in vectors, such as yeast artificial chromosomes and P1s, and to sequence plasmid inserts more rapidly has been developed. A vector primer is combined with single, randomly chosen oligonucleotides in PCRs, to create pools of products. With 12-...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/19962003486
更新日期：1996-03-01 00:00:00
abstract：:DNA-binding proteins can be purified by their affinity for probes with specific sequences that are tethered to magnetic beads. A restriction site at the end of the tether allows release of probe with factor still on it. This probe with bound factor can be bandshifted directly to compare to bandshifts in whole extracts...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1994-05-01 00:00:00
abstract：:Advances in in vitro display and protein engineering yield therapeutics with affinities in the picomolar range. The Gyrolab® microfluidics platform uses the kinetic exclusion assay principle to measure subnanomolar solution affinities. This work describes application of the Gyrolab solution affinity module and the new...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/btn-2020-0080
更新日期：2020-09-01 00:00:00
abstract：:The recently developed ultrasensitive reverse transcriptase (RT) test involving a PCR step can detect minute amounts of RT in single retroviral particles and is 10(6) times more sensitive than conventional RT assays. We have found that different DNA-dependent DNA polymerases like DNA Polymerase I from Escherichia coli...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Metabolic and electrical coupling through gap junction channels is implicated in cell differentiation, tissue homeostasis, and electrotonic propagation of signals in excitable tissues. The characterization of gating properties of these channels requires electrophysiological recordings at both single- and multiple-chan...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/03345rr03
更新日期：2003-05-01 00:00:00
abstract：:Several techniques were evaluated for the quantitation of the total protein content of an IgG2a monoclonal antibody, KS1/4, and its deacetylvinblastine (DAVLB) conjugate. The UV assay is rapid, but it requires an extensive calibration of the response factor, and impurities may cause a high bias. Amino acid analysis (A...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:We have utilized 5-bromo-2'deoxyuridine (BrdU) substituted DNA as a probe for a number of applications including, principally, for chromosome painting by fluorescence in situ hybridization (FISH) but also for DNA end-labeling to detect apoptotic cell death and for filter hybridization. Br-dUTP was used as a substitute...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1996-07-01 00:00:00
abstract：:Thin spun-coat films (~4 nm thick) of graphene oxide (GO) constitute a versatile surface chemistry compatible with a broad range of technologically important sensor materials. Countless publications are dedicated to the nuances of surface chemistries that have been developed for sensors, with almost every material hav...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000114188
更新日期：2014-07-01 00:00:00
abstract：:A brief description of the setup of an inexpensive densitometric analysis system is provided. This system uses a software package and scanner that can be obtained for a total of less than $1000 and that can be operated by any Macintosh computer, including a MacPlus. In the event that a scanner is already available, th...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1994-06-01 00:00:00
abstract：:Characterization of the thermodynamics of binding interactions is important in improving our understanding of bimolecular recognition and forms an essential part of the rational drug design process. Isothermal titration calorimetry (ITC) is rapidly becoming established as the method of choice for undertaking such stud...
journal_title：BioTechniques
pub_type： 杂志文章,评审
doi：
更新日期：2001-07-01 00:00:00
abstract：:Cycling probe technology (CPT) is a simple signal amplification method for the detection of specific target DNA sequences. CPT uses a chimeric DNA-RNA-DNA probe that is cut by RNase H when bound to its complementary target sequence. In this study, a hybridization assay was developed to detect biotinylated CPT products...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/00286st05
更新日期：2000-06-01 00:00:00
abstract：:Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000112003
更新日期：2005-10-01 00:00:00
abstract：:A consensus peptide sequence, QSYP, appears as an artifact during the mapping of monoclonal antibodies (MAbs) using a random peptide phage display library. Phage bearing this QSYP sequence were independently selected by four different laboratories screening separate MAb preparations with the same phage library. In eac...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/03341rr04
更新日期：2003-01-01 00:00:00
abstract：:Previous experiments have clearly demonstrated that microtubule dynamic instability is regulated in living cells, but the molecular mechanisms that are responsible for this regulation are not well understood. We describe two rapid, functional assays that can be used to screen cell extracts for regulators of microtubul...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/98245rr04
更新日期：1998-05-01 00:00:00
abstract：:Determining the primary sequences of informational macromolecules is no longer a limiting factor for our ability to completely understand the biological functioning of cells and organisms. Similarly, our understanding of transcriptional regulation (transcriptomics) has been greatly enhanced by the availability of micr...
journal_title：BioTechniques
pub_type： 杂志文章,评审
doi：10.2144/000113137
更新日期：2009-04-01 00:00:00
abstract：:SOP3 is a web-based software tool for designing oligonucleotide primers for use in the analysis of single nucleotide polymorphisms (SNPs). Accessible via the Internet, the application is optimized for developing the PCR and sequencing primers that are necessary for Pyrosequencing. The application accepts as input gene...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/05381RR01
更新日期：2005-01-01 00:00:00
abstract：:Bionanotechnology is an emerging field in nanotechnology. In general, it uses concepts from chemistry, biochemistry, and molecular biology to identify components and processes for the construction of self-assembling materials and devices. Distant goals of the science of bionanotechnology range from developing programm...
journal_title：BioTechniques
pub_type： 杂志文章,评审
doi：10.2144/04366RV01
更新日期：2004-06-01 00:00:00
abstract：:The Sequence Analysis Workshop (SAW) is an interactive program for sequence analysis of immunoglobulin variable domains. Sequences for SAW can be obtained from GenBank or from a standard text file. SAW can compare a variable domain to as many as 100 different sequences, calculate the extent of homology, sort the seque...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1993-12-01 00:00:00
