Abstract:
:One of the challenges of performing live-cell imaging in plants is establishing a system for securing the sample during imaging that allows for the rapid addition of treatments. Here we report how a commercially available device called a HybriWell™ can be repurposed to create an imaging chamber suitable for Arabidopsis seedlings, cotyledons and leaves. Liquid in the imaging chamber can be rapidly exchanged to introduce chemical treatments via microfluidic passive pumping. When used in conjunction with fluorescent biosensors, this system can facilitate live-cell imaging studies of signal transduction pathways triggered by different treatments. As a demonstration, we show how the HybriWell can be used to monitor flg22-induced calcium transients using the R-GECO1 calcium indicator in detached Arabidopsis leaves.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Vang S,Seitz K,Krysan PJdoi
10.2144/btn-2018-0044subject
Has Abstractpub_date
2018-06-01 00:00:00pages
255-261issue
6eissn
0736-6205issn
1940-9818journal_volume
64pub_type
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