Abstract：
:The CRISPR/Cas9 system is an efficient gene-editing method, but it is difficult to obtain mutants for some specific species and special genome structures. A previously reported multiplexed, semi-nested PCR target-enrichment approach, which does not rely on transgenic technology, has been shown to be an effective and affordable strategy for the discovery of rare mutations in a large sodium azide-induced rice population. However, this strategy has the potential for further optimization. Here, we describe an improved multiplex semi-nested PCR target-enrichment strategy with simplified processing procedures, reduced false-positive rates and increased mutation detection frequency (1 mutation/73 Kb).
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Zhang Y,Chi X,Feng L,Wu X,Qi Xdoi
10.2144/btn-2019-0001subject
Has Abstractpub_date
2019-12-01 00:00:00pages
294-298issue
6eissn
0736-6205issn
1940-9818journal_volume
67pub_type
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