Abstract:
:Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical properties of viruses combined with sequence-independent amplification and cloning. We show that both single- and double-stranded DNA viruses can be recovered from blood samples using this approach. In addition, we report the discovery of novel anellovirus sequences in the blood of healthy donors. PCR primers designed to amplify these novel anellovirus sequences were then used to verify the presence of these viruses in the general donor population.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Breitbart M,Rohwer Fdoi
10.2144/000112019subject
Has Abstractpub_date
2005-11-01 00:00:00pages
729-36issue
5eissn
0736-6205issn
1940-9818pii
000112019journal_volume
39pub_type
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