Abstract:
:A streamlined protocol is described that allows high sensitivity antigen detection by Western blotting in a single day. The choice of membrane blotting matrix, as well as blocking reagents, has been optimized in order to allow rapid development of the blot with chemiluminescent reagents. The entire process, from gel to blot to a permanent, hard copy image on x-ray film, can be accomplished within six hours.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Klapper A,MacKay B,Resh MDsubject
Has Abstractpub_date
1992-05-01 00:00:00pages
650-4issue
5eissn
0736-6205issn
1940-9818journal_volume
12pub_type
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