Abstract：
:An assay is described in which an oligonucleotide probe is specifically digested by lambda exonuclease only when it is annealed to its complementary sequence. In this assay, a cycling effect occurs whereby a small amount of target sequence acts as a specific co-factor in the enzymatic degradation of a larger number of molecules of an oligonucleotide probe. This amplification principle is demonstrated and the effect of the oligonucleotide probe sequence investigated. The necessary steps needed to convert this effect into a useful diagnostic tool are discussed.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Copley CG,Boot Csubject
Has Abstractpub_date
1992-12-01 00:00:00pages
888-92issue
6eissn
0736-6205issn
1940-9818journal_volume
13pub_type
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